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For antibody staining, antigen retrieval was performed on the slides by incubating them in a steamer for 40 min in citrate buffer (Vector Labs, H-3300-250)
Yang JS, Hu Y, Bian J, Yao D, Wang X, Liu Z, Guo S, Zhang L, Peng
Ludwig M
A single injection continues to stimulate GH release throughout the week, creating stable, sustained growth hormone elevation rather than distinct pulses
In addition, crocetin administration reduces follicle loss and rescues fertility in CPM-treated mice
Observable outcomes can be seen in 4-8 weeks based on the treatment procedure and personal skin reaction
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